Klinisch II - P21 - Plasma alemtuzumab levels show great interpatient varia­bility, but are not associated with late acute rejection in simulta­neous pan­creas-kidney recipients

J.R. Bank, M.J.K. Mallat, C.M. Jol-van der Zijde, R.G. Bredius, P.J.M van der Boog, A.E. Braat, J. Ringers, M.E.J. Reinders, H.J.W. de Fijter

Chair(s): drs. Christina Krikke, chirurg, UMC Groningen

Thursday 10 march 2016

13:00 - 13:30h at Foyer

Categories: Best abstracts, Postersessie

Parallel session: Postersessies XI - Opgesplitst in 3 tijdblokken en 3 categoriëen (klinisch, basaal, donatie)

Introduction:
Alemtuzumab induction followed by a steroid-free, tacrolimus (Tac) and mycophenolate mofetil (MMF) based regimen effectively prevented acute rejection after simultaneous pancreas-kidney transplantation (SPKT). Acute rejection was rare in the first three months with a second peak in the cumulative incidence between 3 to 6 months, despite comparable Tac and MMF exposure and similar reconstitution of immune cells in patients with and without rejection. Here, we investigated whether later acute rejections were associated with alemtuzumab plasma levels, since significant interpatient variability has been reported in non-solid organ transplant studies.

Methods:
This retrospective cohort study included 73 consecutive SPKT recipients, all receiving alemtuzumab induction (15 mg subcutaneously, day 0 & 1) and steroid-free maintenance with Tac and MMF. Alemtuzumab levels were measured with ELISA on 3 time points in 10 patients with acute rejection within 6 months and 10 control patients without rejection. Kaplan-Meier estimate (log rank) was used to compare time to undetectable plasma levels.

Results:
A total of 15 patients (20.5%) experienced acute rejection in the first year after SPKT: two patients : Alemtuzumab levels in SPKT recipients showed great interpatient variability, there was however no association between therapeutic drug monitoring parameters and acute rejection episodes beyond 3 months. The observation that late acute rejections were associated intercurrent infections and additional dose reductions, suggests that these empiric reductions in Tac or MMF, in the absence of steroids, constitute an increased risk for late acute rejection in SPKT.